Microinjection and Electroporation

08 - 10 October 2024
FreeAdvance at MRC Harwell
MRC Harwell, Oxfordshire, UK

The Microinjection and Electroporation course provides tailored and hands-on training in embryo harvesting, set up and operation of microinjection rigs, pronuclear and cytoplasmic microinjection and the process of electroporation to introduce CRISPR-Cas9 reagents to embryos.

In addition, this course offers a demonstration of surgical embryo transfer and the opportunity to meet and discuss your specific requirements with experts in IVF technology, CRISPR-Cas9 design and mouse colony management.

Who is this for?

  • Researchers
  • Senior animal technicians
  • PhD students

Following this course, you will have:

  • Experience harvesting and sorting 1-cell and 2-cell embryos
  • An understanding of the use of cryopreserved embryos and IVF embryos in gene delivery
  • Practical training in pronuclear and cytoplasmic injection of CRISPR-Cas9 reagent into the embryo
  • Practical training of embryo electroporation
  • An understanding of the principles of colony management, IVF and cryopreservation

Cost: 1100£

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